加州鲈Caspase-3基因克隆及表达分析

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安徽农学通报 ›› 2023, Vol. 29 ›› Issue (21) : 73-79.

动物科学

加州鲈Caspase-3基因克隆及表达分析

梁富, 宋长江, 唐怀庆, 丘金珠, 赵天珍, 郑汉生, 宋海霞^*

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Cloning and expression analysis of Caspase-3 in Micropterus salmoides

LIANG Fu, SONG Changjiang, TANG Huaiqing, QIU Jinzhu, ZHAO Tianzhen, ZHENG Hansheng, SONG Haixia^*

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摘要

从加州鲈（Micropterus salmoides）头肾组织中克隆获得Caspase家族中Caspase-3基因的编码区序列,通过生物信息学分析工具分析Caspase-3基因的蛋白结构,预测其功能并检测其在加州鲈各组织中的表达量。结果表明,该基因cDNA全长890 bp,ORF为852 bp,可编码283个氨基酸,蛋白理论分子质量为73.06 ku,理论等电点（PI）为5.09;Caspase-3基因含有一个保守信号肽序列,不存在跨膜区;多序列比对结果显示加州鲈Caspase-3与红笛鲷（Lutjanus peru）Caspase-3相似度最高,高达88.38%;系统进化分析发现加州鲈Caspase-3与红笛鲷聚为一支;Caspase-3基因在所取组织中均有表达,在脑组织中表达量最高,在鳃组织中表达量最低。

Abstract

The sequence of the coding region of Caspase-3 gene in Caspase family was obtained from the head and kidney tissue of Micropterus salmoides, and the protein structure and function of Caspase-3 gene was analyzed by bioinformatics analysis tools . The results showed that the cDNA of Caspase-3 was 890 bp, ORF was 852 bp, encoding 283 amino acids. The theoretical molecular mass was 73.06 ku, and the theoretical isoelectric point (PI) was 5.09. This gene contained a conserved signal peptide sequence without the transmembrane region. Multiple sequence alignment showed that the M. salmoides Caspase-3 met Lutjanus peru Caspase-3 up to 88.38%. Phylogenetic analysis found that M. salmoides Caspase-3 and L. peru cluster together. The Caspase-3 gene was expressed in the selected tissues, which was the highest in brain tissues and the lowest in gill tissues.

导出引用

梁富, 宋长江, 唐怀庆, 丘金珠, 赵天珍, 郑汉生, 宋海霞. 加州鲈Caspase-3基因克隆及表达分析[J]. 安徽农学通报, 2023, 29(21): 73-79.

LIANG Fu, SONG Changjiang, TANG Huaiqing, QIU Jinzhu, ZHAO Tianzhen, ZHENG Hansheng, SONG Haixia. Cloning and expression analysis of Caspase-3 in Micropterus salmoides[J]. Anhui Agricultural Science Bulletin, 2023, 29(21): 73-79.

中图分类号： S917.4 S9-9

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中山市社会公益与基础研究项目（2021B2056）

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收稿日期	出版日期
2023-09-04	2023-11-15
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2023-12-21

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