To screen soybean varieties suitable for planting in Jiaxiang County, Shandong Province, a regional comparative trial was conducted using 14 varieties, including Hedou 12, Jiadou 119, and Daoqiu 60, with Hedou 12 as the control (CK). The indicators such as growth period, agronomic traits, economic traits, disease resistance, yield, and quality were measured. The results showed that in the 2022 regional trial, the growth period of the varieties ranged from 92.6 to 107.9 days, plant height from 56.4 to 84.3 cm, effective pods per plant from 40.4 to 68.9, grains per plant from 86.8 to 139.8, grain weight per plant from 20.2 to 28.9 g, and 100-seed weight from 17.4 to 29.1 g. Traits such as grain shape, seed coat color, seed luster, hilum color, purple-brown spot rate, and insect damage rate all performed well. Except for Xiangxing 4 and Xiangxing 919, all other varieties exhibited resistance or high resistance to soybean mosaic virus strains SC-3 and SC-7, the yields were 180.80 - 229.08 kg/667 m². Based on the 2022 regional trial, Shengdi 16, Daoqiu 60, Jiadou 119, and Jundou 12 were selected for their moderate growth period, excellent agronomic and economic traits, and higher yields, and were advanced to the 2023 regional trial. The two year regional trial results showed that Shengdi 16, Daoqiu 60, Jiadou 119, and Jundou 12 exhibited high and stable yields, with yields over the two years increasing by 5.06% to 6.08% compared to CK. Their protein content ranged from 39.83% to 41.54%, and crude fat content from 18.98% to 20.97%. In conclusion, Shengdi 16, Daoqiu 60, Jiadou 119, and Jundou 12 demonstrated superior overall performance and were recommended for production trials.